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Dog Dopamine(DA) ELISA Kit

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    Negotiable

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  • Delivery term:

    The date of payment from buyers deliver within days

  • seat:

    Zhejiang

  • Validity to:

    Long-term effective

  • Last update:

    2017-11-01 05:47

  • Browse the number:

    171

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Sunlong Biotech Co.,Ltd
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shenglong(Mr.)  

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Zhejiang

Address:

Yufeng Building, No.333, Pingshui Road, Hangzhou, Zhejiang, China

Website: http://www.online-biotech.com/ http://shenglong.prepaidcashbackcards.com/
Product details
Product Name: Dog dopamine(DA) ELISA kit
Product Type: ELISA Kit
Code : CSB-EQ027496DO
Size : 96T,5×96T,10×96T
Abbreviation: DA
Species : Dog
Sample Types: serum, plasma and tissue homogenates
Detect Range: 37.5 pg/ml-600 pg/ml
Sensitivity: 37.5 pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450nm
Research Area: Neuroscience
Protocol :
Instructions
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :

Intra-assay Precision (Precision within an assay): CV%<15

%Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.
Linearity :

To assess the linearity of the assay, samples were spiked with high concentrations of dog DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.                                        

                    Sample              Serum(n=4)

1:1              Average %               92

                 Range %                90-97

1:2              Average %               95

                 Range %                 88-100

1:4               Average %              95

                  Range %                89-100 

1:8              Average %               92

                  Range %                86-99
Recovery :

The recovery of dog DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type             Average % Recovery        Range

Serum (n=5)                 94                              89-100

EDTA plasma (n=4)             95                       85-105
Typical Data:

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml     OD1      OD2        Average

600      0.296    0.294       0.295 

300      0.524    0.532       0.528 

150      0.905    0.901       0.903

75       1.724    1.763       1.744

37.5     2.543    2.588       2.566

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